Composite

Part:BBa_K2180004

Designed by: Sofiane Safi-Stibler   Group: iGEM16_UPMC-Paris   (2016-08-05)


Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator

This is the assembly of our basic parts BBa_K2180009 , BBa_K2180007 and BBa_K2180008.
The NcoI restriction site is overlapping parts BBa_K2180009 and BBa_K2180007 at the Start codon of LacI + LVA.
This part is an adaptation for B. subtilis of the biobrick BBa_K1163999 from iGEM13_Evry (2013-09-02):
This system allow the synthese of the protein Lac I in the absence of Iron.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
    Illegal AgeI site found at 1165
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None